gephyrin Polyclonal antibody
gephyrin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 12681-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 2 publications below |
Product Information
12681-1-AP targets gephyrin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | gephyrin fusion protein Ag3370 相同性解析による交差性が予測される生物種 |
| Full Name | gephyrin |
| Calculated molecular weight | 769 aa, 83 kDa |
| Observed molecular weight | 93 kDa |
| GenBank accession number | BC030016 |
| Gene symbol | GPHN |
| Gene ID (NCBI) | 10243 |
| RRID | AB_2247770 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for gephyrin antibody 12681-1-AP | Download protocol |
| IHC protocol for gephyrin antibody 12681-1-AP | Download protocol |
| IF protocol for gephyrin antibody 12681-1-AP | Download protocol |
| IP protocol for gephyrin antibody 12681-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neurosci Microglial Tmem59 Deficiency Impairs Phagocytosis of Synapse and Leads to Autism-Like Behaviors in Mice. | ||
Cell Rep Optogenetic Activation of Striatopallidal Neurons Reveals Altered HCN Gating in DYT1 Dystonia. | ||
Neurosci Lett Microglia participate in postoperative cognitive dysfunction by mediating the loss of inhibitory synapse through the complement pathway | ||
Arthritis Rheumatol Anti-Gephyrin antibodies: A novel specificity in patients with systemic sclerosis and lower bowel dysfunction | ||
Adv Funct Mater Metabolically Glycoengineered Neural Stem Cells Boost Neural Repair After Cardiac Arrest |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Reyes (Verified Customer) (03-01-2024) | Gephyrin antibody (in red) works on human brain paraffin sections, although being found in the synapsis would make it difficult to quantify in the tissue.
 |
FH Deria (Verified Customer) (07-14-2022) | This antibody works fine
|
