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GPI Polyclonal antibody

Name: GPI Polyclonal antibody
Brand: Proteintech
SKU: 15171-1-AP
Rating: 5.00 (3 reviews)

GPI Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Reviews (3)

Cat no : 15171-1-AP

Synonyms

NLK, Neuroleukin, GNPI, Glucose-6-phosphate isomerase, EC:5.3.1.9

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 15171-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15171-1-AP
WB result of GPI antibody (15171-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells.
WB analysis using 15171-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15171-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15171-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15171-1-AP
WB result of GPI antibody (15171-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells.
IHC staining of human lung cancer using 15171-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15171-1-AP (GPI Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15171-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15171-1-AP (GPI Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 15171-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15171-1-AP (GPI antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of PC-3 using 15171-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 15171-1-AP (GPI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 15171-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, PC-3 cells, PC3 cells, U251 cells, U87-MG cells
Positive IHC detected in	human lung cancer tissue, human normal colon **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 21 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

15171-1-AP targets GPI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GPI fusion protein Ag7423 相同性解析による交差性が予測される生物種
Full Name	glucose phosphate isomerase
Calculated molecular weight	63 kDa
Observed molecular weight	55-64 kDa
GenBank accession number	BC004982
Gene symbol	GPI
Gene ID (NCBI)	2821
RRID	AB_2263537
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).

Protocols

Product Specific Protocols
WB protocol for GPI antibody 15171-1-AP	Download protocol
IHC protocol for GPI antibody 15171-1-AP	Download protocol
IF protocol for GPI antibody 15171-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P Authors - Yuan-Li Ai View Article
mouse	IF	Nat Neurosci A glycolytic shift in Schwann cells supports injured axons. Authors - Elisabetta Babetto View Article
human	WB	Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Authors - Linchong Sun View Article
mouse	WB	EMBO J Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission. Authors - Lihua Wang View Article
human	WB	EMBO Rep CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis. Authors - Xiuying Zhong View Article
human	WB	EMBO Rep OMA1 reprograms metabolism under hypoxia to promote colorectal cancer development. Authors - Zhida Wu View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Marco (Verified Customer) (07-04-2024)	A great antibody for NRVCMs Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: NRVCMs
FH Kristian (Verified Customer) (07-27-2021)	GPI is shown in green. Nuclei are shown in blue. Applications: Immunofluorescence Primary Antibody Dilution: 1:200 Cell Tissue Type: RCC4 GPI Antibody Immunofluorescence validation (1:200 dilution) in RCC4 (Cat no:15171-1-AP)
FH Daniel (Verified Customer) (02-06-2020)	Denatured lysate was separated on a 4-20% PAGE Applications: Western Blot, Primary Antibody Dilution: 1:2000 Cell Tissue Type: mouse kidney GPI Antibody Western Blot, validation (1:2000 dilution) in mouse kidney (Cat no:15171-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPI Polyclonal antibody

GPI Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 15171-1-AP

WB analysis of HeLa using 15171-1-AP

WB analysis using 15171-1-AP

WB analysis of PC-3 using 15171-1-AP

WB analysis of HeLa using 15171-1-AP

WB analysis of HeLa using 15171-1-AP

IHC staining of human lung cancer using 15171-1-AP

IHC staining of human lung cancer using 15171-1-AP

IHC staining of human normal colon using 15171-1-AP

IF Staining of PC-3 using 15171-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

A glycolytic shift in Schwann cells supports injured axons.

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.

OMA1 reprograms metabolism under hypoxia to promote colorectal cancer development.

Reviews