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  • Featured Product
  • KD/KO Validated

GPI Monoclonal antibody

GPI Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F11E4

Cat no : 67178-1-Ig

Synonyms

AMF, Autocrine motility factor, Glucose 6 phosphate isomerase, glucose phosphate isomerase, GNPI, GPI, Neuroleukin, NLK, PGI, PHI, Phosphoglucose isomerase, Phosphohexose isomerase, SA 36, Sperm antigen 36

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPI Monoclonal antibody (67178-1-Ig)

    WB analysis using 67178-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67178-1-Ig (GPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GPI Monoclonal antibody (67178-1-Ig)

    WB analysis of HeLa using 67178-1-Ig

    WB result of GPI antibody (67178-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using GPI Monoclonal antibody (67178-1-Ig)

    WB analysis of HeLa using 67178-1-Ig

    WB result of GPI antibody (67178-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GPI Monoclonal antibody (67178-1-Ig)

    IHC staining of human lung cancer using 67178-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67178-1-Ig (GPI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GPI Monoclonal antibody (67178-1-Ig)

    IHC staining of human lung cancer using 67178-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67178-1-Ig (GPI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using GPI Monoclonal antibody (67178-1-Ig)

    IF Staining of PC-3 using 67178-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using 67178-1-Ig (GPI antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, PC-3 cells, LNCaP cells, HEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67178-1-Ig targets GPI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPI fusion protein Ag7419 相同性解析による交差性が予測される生物種
Full Name glucose phosphate isomerase
Calculated molecular weight 63 kDa
Observed molecular weight 55-64 kDa
GenBank accession numberBC004982
Gene symbol GPI
Gene ID (NCBI) 2821
RRIDAB_2882474
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).

Protocols

Product Specific Protocols
WB protocol for GPI antibody 67178-1-IgDownload protocol
IHC protocol for GPI antibody 67178-1-IgDownload protocol
IF protocol for GPI antibody 67178-1-IgDownload protocol
Standard Protocols
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