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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPNMB Polyclonal antibody (27037-1-AP)

    WB analysis using 27037-1-AP

    TT cells were subjected to SDS PAGE followed by western blot with 27037-1-AP (GPNMB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L-929 cells using GPNMB Polyclonal antibody (27037-1-AP)

    WB analysis of L-929 using 27037-1-AP

    L-929 cells were subjected to SDS PAGE followed by western blot with 27037-1-AP (GPNMB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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GPNMB Polyclonal antibody

GPNMB Polyclonal Antibody for WB, ELISA
Cat No. 27037-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

UNQ1725/PRO9925, Transmembrane glycoprotein NMB, Osteoactivin, NMB, Hematopoietic growth factor inducible neurokinin-1 type

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTT cells, L-929 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27037-1-AP targets GPNMB in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPNMB fusion protein Ag25826 相同性解析による交差性が予測される生物種
Full Name glycoprotein (transmembrane) nmb
Calculated molecular weight 64 kDa
Observed molecular weight120 kDa, 95 kDa
GenBank accession numberBC011595
Gene Symbol GPNMB
Gene ID (NCBI) 10457
RRIDAB_3669581
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14956
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.

Protocols

Product Specific Protocols
WB protocol for GPNMB antibody 27037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols