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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPNMB Monoclonal antibody, PBS Only (Capture)

GPNMB Monoclonal Antibody for Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H3E12

Cat no : 66926-2-PBS

Synonyms

GPNMB, HGFIN, Transmembrane glycoprotein NMB

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50045-1

    Sandwich ELISA standard curve of MP50045-1

    Sandwich ELISA standard curve of MP50045-1, GPNMB Monoclonal Matched Antibody Pair - PBS only. 66926-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag26747. 66926-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sandwich ELISA standard curve of MP50045-2

    Sandwich ELISA standard curve of MP50045-2

    Sandwich ELISA standard curve of MP50045-2, GPNMB Monoclonal Matched Antibody Pair - PBS only. 66926-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag26747. 66926-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66926-2-PBS targets GPNMB as part of a matched antibody pair:

MP50045-1: 66926-2-PBS capture and 66926-4-PBS detection (validated in Sandwich ELISA)

MP50045-2: 66926-2-PBS capture and 66926-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.03 x 10-10M
KOff=1.53 x 10-6M
KOn=1.49 x 104M
Immunogen GPNMB fusion protein Ag26747 相同性解析による交差性が予測される生物種
Full Name glycoprotein (transmembrane) nmb
Calculated molecular weight 64 kDa
GenBank accession numberBC011595
Gene symbol GPNMB
Gene ID (NCBI) 10457
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.