• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of THP-1 cells using GPNMB Recombinant antibody (84160-3-RR)

    WB analysis of THP-1 using 84160-3-RR (same clone as 84160-3-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 84160-3-RR (GPNMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84160-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPNMB Recombinant antibody (84160-3-RR)

    WB analysis using 84160-3-RR (same clone as 84160-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84160-3-RR (GPNMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84160-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01096-1

    Sandwich ELISA standard curve of MP01096-1

    Sandwich ELISA standard curve of MP01096-1, Human GPNMB Recombinant Matched Antibody Pair - PBS only. 84160-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14124. 84160-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • Sample test of MP01096-1

    Sample test of MP01096-1

    Plasma of six individual healthy human donors was measured. The GPNMB concentration of detected samples was determined to be 12.18 ng/mL with a range of 3.43-30.61 ng/mL.

  • Sample test of MP01096-1

    Sample test of MP01096-1

    Serum of six individual healthy human donors was measured. The GPNMB concentration of detected samples was determined to be 5.69 ng/mL with a range of 3.96-8.17 ng/mL.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GPNMB Recombinant antibody (84160-3-RR)

    IF Staining of HeLa using 84160-3-RR (same clone as 84160-3-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPNMB antibody (84160-3-RR, Clone: 241435F3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84160-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01096-1

    Cytometric bead array standard curve of MP01096-1

    Cytometric bead array standard curve of MP01096-1, GPNMB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84160-3-PBS. Detection antibody: 84160-2-PBS. Standard: Ag14124. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84160-3-RR

    Affinity and Kinetic Characterization of 84160-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84160-3-RR against Human GPNMB were performed. The affinity constant is below 1 pM.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

GPNMB Recombinant antibody, PBS Only (Capture)

GPNMB Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84160-3-PBS
Clone No.241435F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

UNQ1725/PRO9925, Transmembrane glycoprotein NMB, Osteoactivin, NMB, Hematopoietic growth factor inducible neurokinin-1 type

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84160-3-PBS targets GPNMB as part of a matched antibody pair:

MP01096-1: 84160-3-PBS capture and 84160-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GPNMB fusion protein Ag14124 相同性解析による交差性が予測される生物種
Full Name glycoprotein (transmembrane) nmb
Calculated molecular weight 64 kDa
Observed molecular weight120 kDa, 95 kDa
GenBank accession numberBC011595
Gene Symbol GPNMB
Gene ID (NCBI) 10457
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14956
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.