Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, mouse cerebellum tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
25076-1-AP targets GPR107 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GPR107 fusion protein Ag18649 相同性解析による交差性が予測される生物種 |
| Full Name | G protein-coupled receptor 107 |
| Calculated molecular weight | 552 aa, 67 kDa |
| Observed molecular weight | 60-67 kDa |
| GenBank accession number | BC110518 |
| Gene Symbol | GPR107 |
| Gene ID (NCBI) | 57720 |
| RRID | AB_2879886 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VW38 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GPR107 also named as KIAA1624 or LUSTR1 is 600 amino acid protein, which belongs to the LU7TM family. GPR107 localizes in membrane and is a multi-pass membrane protein. GPR107 is a promising candidate receptor for neuronostatin, and neuronostatin, interacting with GPR107, may play an important role in the central control of cardiovascular function.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR107 antibody 25076-1-AP | Download protocol |
| IF protocol for GPR107 antibody 25076-1-AP | Download protocol |
| FC protocol for GPR107 antibody 25076-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Chem Biol A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes. | ||
J Exp Clin Cancer Res LncGPR107 drives the self-renewal of liver tumor initiating cells and liver tumorigenesis through GPR107-dependent manner. | ||
Neurochem Res Neuronostatin Promotion Soluble Aβ1-42 Oligomers: Induced Dysfunctional Brain Glucose Metabolism in Mice. | ||
Behav Brain Res Neuronostatin promotes soluble Aβ1-42 oligomers -induced spatial learning and memory impairments in mice.
| ||
Nat Chem Biol Chemoproteomics reveals microbiota-derived aromatic monoamine agonists for GPRC5A | ||
Neuropharmacology Neuronostatin regulates neuronal function and energetic metabolism in Alzheimer's disease in a GPR107-dependent manner
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