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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse colon tissue using GPR112 Polyclonal antibody (31781-1-AP)

    IHC staining of mouse colon using 31781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31781-1-AP (GPR112 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using GPR112 Polyclonal antibody (31781-1-AP)

    IHC staining of mouse colon using 31781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31781-1-AP (GPR112 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using GPR112 Polyclonal antibody (31781-1-AP)

    IF Staining of RAW 264.7 using 31781-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using GPR112 antibody (31781-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

GPR112 Polyclonal antibody

GPR112 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 31781-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

RP1 299I16, PGR17, G protein coupled receptor 112

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31781-1-AP targets GPR112 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR112 fusion protein Ag36114 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 112
Calculated molecular weight333kDa
GenBank accession numberNM_153834
Gene Symbol GPR112
Gene ID (NCBI) 139378
RRIDAB_3670112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8IZF6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPR112 is a member of the adhesion G protein-coupled receptor (aGPCR) family. These receptors are characterized by a complex architecture and large size, mainly attributed to their N terminus, which contains a variety of domains involved in cell-cell and cell-matrix interactions. The function of GPR112 and other aGPCRs is closely related to key aspects of the development, architecture, and function of the nervous system. They are involved in neuronal migration, axon guidance, dendritogenesis, and synaptogenesis. Additionally, aGPCRs, including GPR112, have been implicated in the development of myelinating glial cells, which are crucial for proper neuronal signal propagation.

Protocols

Product Specific Protocols
IHC protocol for GPR112 antibody 31781-1-APDownload protocol
IF protocol for GPR112 antibody 31781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols