Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Y79 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 3 publications below |
Product Information
14047-1-AP targets GPR116 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GPR116 fusion protein Ag5214 相同性解析による交差性が予測される生物種 |
| Full Name | G protein-coupled receptor 116 |
| Calculated molecular weight | 1346 aa, 149 kDa |
| Observed molecular weight | 149 kDa |
| GenBank accession number | BC066121 |
| Gene Symbol | GPR116 |
| Gene ID (NCBI) | 221395 |
| RRID | AB_2113095 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IZF2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GPR116 (ADGRF5, adhesion G protein-coupled receptor F5) is involved in the G protein-coupled receptor signaling pathway and cell surface receptor signaling pathway. may act upstream of or within several processes, including glomerular filtration; pharyngeal arch artery morphogenesis; and surfactant homeostasis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR116 antibody 14047-1-AP | Download protocol |
| IHC protocol for GPR116 antibody 14047-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncotarget High expression of GPR116 indicates poor survival outcome and promotes tumor progression in colorectal carcinoma. | ||
Medicine (Baltimore) GPR116 overexpression correlates with poor prognosis in gastric cancer. |