Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse small intestine tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
20050-1-AP targets GPR124 in IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | G protein-coupled receptor 124 |
Calculated molecular weight | 143 kDa |
GenBank accession number | NM_032777 |
Gene Symbol | GPR124 |
Gene ID (NCBI) | 25960 |
RRID | AB_3669320 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96PE1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GPR124, also named KIAA1531 and TEM5, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. GPR124 is an Orphan receptor. GPR124 is a Tumor endothelial marker (TEM) that is significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. GPR124 is a member of the adhesion family of G protein-coupled receptors and is localized on the surface of endothelial cells. GPR124 is a seven-pass transmembrane receptor, unlike TEM1, TEM7, and TEM8 which span the membrane once. Proteolytically processed soluble GPR124 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans. The antibody recognizes near the C-term of GPR124. The antibody can't recognize the isoform3 of GPR124.
Protocols
Product Specific Protocols | |
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IHC protocol for GPR124 antibody 20050-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |