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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPR155 Polyclonal antibody

GPR155 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12659-1-AP

Synonyms

DEP.7, DEPDC3, G protein coupled receptor 155, GPR155, LYCHOS, PGR22

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPR155 Polyclonal antibody (12659-1-AP)

    WB analysis using 12659-1-AP

    mouse brain lysates were subjected to SDS PAGE followed by western blot with 12659-1-AP (GPR155 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GPR155 Polyclonal antibody (12659-1-AP)

    IP experiment of mouse brain using 12659-1-AP

    IP result of anti-GPR155 (IP:12659-1-AP, 4ug; Detection:12659-1-AP 1:300) with mouse brain tissue lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using GPR155 Polyclonal antibody (12659-1-AP)

    IHC staining of human brain using 12659-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using GPR155 Polyclonal antibody (12659-1-AP)

    IHC staining of human brain using 12659-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GPR155 Polyclonal antibody (12659-1-AP)

    IHC staining of mouse brain using 12659-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GPR155 Polyclonal antibody (12659-1-AP)

    IHC staining of mouse brain using 12659-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12659-1-AP targets GPR155 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR155 fusion protein Ag3328 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 155
Calculated molecular weight 870 aa, 97 kDa
Observed molecular weight 97 kDa, 75 kDa
GenBank accession numberBC028730
Gene symbol GPR155
Gene ID (NCBI) 151556
RRIDAB_2877869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The G Protein-coupled receptor 155 (GPR155), also known as Lysosomal cholesterol signaling protein (LYCHOS), is a cholesterol receptor on the lysosome. GRR155 plays a role in the cholesterol-sensing lysosomal pathway and couples cholesterol concentration to MTORC1-dependent anabolic signaling (PMID: 36007018). GPR155 may represent a biomarker for diagnosing and predicting hematogenous metastasis of gastric cancer (PMID: 28165032).

Protocols

Product Specific Protocols
WB protocol for GPR155 antibody 12659-1-APDownload protocol
IHC protocol for GPR155 antibody 12659-1-APDownload protocol
IP protocol for GPR155 antibody 12659-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Pathol Res Pract

Circular RNA hsa_circ_0094976 modulates GPR155 to inhibit gastric adenocarcinoma malignant characteristics by targeting miR-223-3p

Authors - Li Liu
View Article