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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPR158 Polyclonal antibody (31846-1-AP)

    WB analysis using 31846-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31846-1-AP (GPR158 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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GPR158 Polyclonal antibody

GPR158 Polyclonal Antibody for WB, ELISA
Cat No. 31846-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Probable G-Protein Coupled Receptor 158, mGlyR, Metabotropic glycine receptor, KIAA1136, G-protein coupled receptor 158

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31846-1-AP targets GPR158 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR158 fusion protein Ag34602 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 158
Calculated molecular weight135 kDa
Observed molecular weight150 kDa
GenBank accession numberBC157124
Gene Symbol GPR158
Gene ID (NCBI) 57512
RRIDAB_3670127
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ5T848
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

G protein-coupled receptors (GPCR) are the largest family of proteins encoded in mammalian genomes that detect extracellular signals to program cellular response, which are essential to understanding physiology, disease, and drug development (PMID: 29249361; 29298813). G protein-coupled receptor 158 (GPR158, also known as mGlyR) is a class C orphan GPCR prominently expressed in brain tissue, where it controls synapse formation and function (PMID: 22689652; 34793198). It can transduce signals by coupling to the regulator of protein signaling (RGS) proteins and be related to the development of major depressive disorder and prostate cancer (PMID: 36996198; 29419376; 25693195).

Protocols

Product Specific Protocols
WB protocol for GPR158 antibody 31846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols