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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPR161 Polyclonal antibody (29328-1-AP)

    WB analysis using 29328-1-AP (same clone as 29328-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29328-1-AP (GPR161 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29328-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of ARPE-19 cells using GPR161 Polyclonal antibody (29328-1-AP)

    IF Staining of ARPE-19 using 29328-1-AP (same clone as 29328-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using GPR161 antibody (29328-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 29328-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using GPR161 Polyclonal antibody (29328-1-AP)

    IF Staining of hTERT-RPE1 using 29328-1-AP (same clone as 29328-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using GPR161 antibody (29328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 29328-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of ARPE-19 cells using GPR161 Polyclonal antibody (29328-1-AP)

    IF Staining of ARPE-19 using 29328-1-AP (same clone as 29328-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using GPR161 antibody (29328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 29328-1-PBS in a different storage buffer formulation.

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GPR161 Polyclonal antibody, PBS Only

GPR161 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 29328-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29328-1-PBS targets GPR161 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR161 fusion protein Ag30998 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 161
Calculated molecular weight 529 aa, 59 kDa
Observed molecular weight70 kDa
GenBank accession numberBC028163
Gene Symbol GPR161
Gene ID (NCBI) 23432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N6U8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays an important role in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms (PMID: 26305592). Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In the presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. GPR161 has a calculated molecular mass of 59 kDa. The 70-kDa band detected by this antibody probably represents a modified version of GPR161 (PMID: 18250320).