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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPR162 Polyclonal antibody

GPR162 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15254-1-AP

Synonyms

A 2, G protein coupled receptor 162, GPR162, GRCA

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using GPR162 Polyclonal antibody (15254-1-AP)

    WB analysis of SH-SY5Y using 15254-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using GPR162 Polyclonal antibody (15254-1-AP)

    WB analysis of Y79 using 15254-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using GPR162 Polyclonal antibody (15254-1-AP)

    WB analysis of human brain using 15254-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using GPR162 Polyclonal antibody (15254-1-AP)

    WB analysis of human brain using 15254-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GPR162 Polyclonal antibody (15254-1-AP)

    WB analysis of HeLa using 15254-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inSH-SY5Y cells, HeLa cells, human brain tissue, Y79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15254-1-AP targets GPR162 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR162 fusion protein Ag7011 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 162
Calculated molecular weight 64 kDa
Observed molecular weight 33 kDa, 64 kDa
GenBank accession numberBC002353
Gene symbol GPR162
Gene ID (NCBI) 27239
RRIDAB_10665369
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GPR162 antibody 15254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Acta Neuropathol Commun

Blood-based CNS regionally and neuronally enriched extracellular vesicles carrying pTau217 for Alzheimer's disease diagnosis and differential diagnosis

Authors - Zhen Guo
View Article