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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPR183 Polyclonal antibody (32243-1-AP)

    WB analysis using 32243-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32243-1-AP (GPR183 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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GPR183 Polyclonal antibody

GPR183 Polyclonal Antibody for WB, ELISA
Cat No. 32243-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EBI2, GPR183/EBI2, GP183, G protein coupled receptor 183, Epstein-Barr virus-induced G-protein coupled receptor 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32243-1-AP targets GPR183 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR183 fusion protein Ag36765 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 183
Calculated molecular weight 361 aa, 41 kDa
Observed molecular weight50 kDa
GenBank accession numberBC020752
Gene Symbol EBI2
Gene ID (NCBI) 1880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP32249
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

G-protein-coupled receptor 183, also known as Epstein-Barr virus-induced gene 2 (EBI2), was initially identified in B cells and is the most upregulated gene after infection with Epstein-Barr virus (PMID:37353188). GPR183 is known to play a key role in the development of antibody responses in secondary lymphoid organs (PMID:23473835).

Protocols

Product Specific Protocols
WB protocol for GPR183 antibody 32243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols