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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using GPR37/Pael-R Polyclonal antibody (14820-1-AP)

    WB analysis of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using GPR37/Pael-R Polyclonal antibody (14820-1-AP)

    WB analysis of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using GPR37/Pael-R Polyclonal antibody (14820-1-AP)

    WB analysis of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GPR37/Pael-R Polyclonal antibody (14820-1-AP)

    IHC staining of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GPR37/Pael-R Polyclonal antibody (14820-1-AP)

    IHC staining of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14820-1-AP (GPR37/Pael-R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GPR37/Pael-R Polyclonal antibody (14820-1-AP)

    IF Staining of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GPR37/Pael-R Polyclonal antibody (14820-1-AP)

    IF Staining of mouse brain using 14820-1-AP (same clone as 14820-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GPR37/Pael-R Polyclonal antibody (14820-1-AP)

    IF Staining of HeLa using 14820-1-AP (same clone as 14820-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPR37/Pael-R antibody (14820-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14820-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GPR37/Pael-R Polyclonal antibody, PBS Only

GPR37/Pael-R Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 14820-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

GPR37, Pael-R, G-protein coupled receptor 37, ETBR-LP-1, ETBR LP 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14820-1-PBS targets GPR37/Pael-R in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR37/Pael-R fusion protein Ag6591 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 37 (endothelin receptor type B-like)
Calculated molecular weight 67 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC040007
Gene Symbol GPR37
Gene ID (NCBI) 2861
RRIDAB_2232549
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15354
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The orphan G protein-coupled receptor GPR37, also known as PAELR (parkin-associated endothelin receptor-like receptor) or ETBR-LP-1 (endothelin B receptor-like protein 1), is a 613 aa, multi-pass membrane protein predominantly expressed in the brain. It is a substrate of parkin (PARK2). When overexpressed in cells, GPR37 tends to become insoluble, unfolded, and ubiquitinated in vivo. Accumulation of the unfolded protein may lead to dopaminergic neuronal death in juvenile Parkinson disease (JPD). This antibody recognizes endogenous GPR37, which migrates as an approximately 50-55 kDa band in SDS-PAGE (PMID:17519329).