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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPR4 Polyclonal antibody

GPR4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 28232-1-AP

Synonyms

G protein coupled receptor 19, G protein coupled receptor 4, GPR19, GPR4

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse lung tissue using GPR4 Polyclonal antibody (28232-1-AP)

    IHC staining of mouse lung using 28232-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28232-1-AP (GPR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GPR4 Polyclonal antibody (28232-1-AP)

    IHC staining of mouse kidney using 28232-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28232-1-AP (GPR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse lung tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28232-1-AP targets GPR4 in IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR4 fusion protein Ag28273 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 4
Calculated molecular weight 362 aa, 41 kDa
GenBank accession numberBC067535
Gene symbol GPR4
Gene ID (NCBI) 2828
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPR4 (G-protein-coupled receptor 4) is a proton-sensing G-protein-coupled receptor (GPCR) that contributes to neovascularization in acidic microenvironments (PMID: 33632325). GPR4 is highly expressed in vascular endothelial cells (ECs) and blood vessel-rich tissues such as the lung, kidney, heart, and liver. Moreover, GPR4 has been shown to mediate EC inflammatory responses to acidosis and is central for leukocyte-endothelium interaction (PMID: 27940273).

Protocols

Product Specific Protocols
IHC protocol for GPR4 antibody 28232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols