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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using GPR52 Polyclonal antibody (30863-1-AP)

    IHC staining of mouse small intestine using 30863-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30863-1-AP (GPR52 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GPR52 Polyclonal antibody (30863-1-AP)

    IHC staining of mouse brain using 30863-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30863-1-AP (GPR52 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GPR52 Polyclonal antibody (30863-1-AP)

    IF Staining of HepG2 using 30863-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPR52 antibody (30863-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

GPR52 Polyclonal antibody

GPR52 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 30863-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

G protein coupled receptor 52

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse small intestine tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30863-1-AP targets GPR52 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR52 fusion protein Ag31188 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 52
Calculated molecular weight 361 aa, 41 kDa
GenBank accession numberBC067456
Gene Symbol GPR52
Gene ID (NCBI) 9293
RRIDAB_3669768
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2T5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPR52, or G protein-coupled receptor 52, is an orphan G protein-coupled receptor (GPCR) that constitutively increases cellular cAMP levels and is coupled to the Gs protein, which activates adenylate cyclase. Agonism of GPR52 has been proposed as a therapeutic intervention for the psychotic and cognitive aspects of schizophrenia. Its role in modulating neurotransmission and the identification of ligands that can interact with it highlight its potential in therapeutic development.

Protocols

Product Specific Protocols
IHC protocol for GPR52 antibody 30863-1-APDownload protocol
IF protocol for GPR52 antibody 30863-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols