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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GPR56 Recombinant antibody (84915-1-RR)

    WB analysis of HeLa using 84915-1-RR (same clone as 84915-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 84915-1-RR (GPR56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84915-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01673-1

    Sandwich ELISA standard curve of MP01673-1

    Sandwich ELISA standard curve of MP01673-1, Human GPR56 Recombinant Matched Antibody Pair - PBS only. 84915-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2478. 84915-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01673-1

    Sample test of MP01673-1

    The mean GPR56 concentration was determined to be 5.01 ng/mL in A549 cell extract based on a 1.50 mg/mL extract load and 5.18 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load.

  • Cytometric bead array standard curve of MP01673-2

    Cytometric bead array standard curve of MP01673-2

    Cytometric bead array standard curve of MP01673-2, GPR56 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84915-1-PBS. Detection antibody: 84915-3-PBS. Standard: Eg2478. Range: 0.781-50 ng/mL.

  • Cytometric bead array standard curve of MP01673-1

    Cytometric bead array standard curve of MP01673-1

    Cytometric bead array standard curve of MP01673-1, GPR56 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84915-1-PBS. Detection antibody: 84915-2-PBS. Standard: Eg2478. Range: 0.781-50 ng/mL.

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GPR56 Recombinant antibody, PBS Only (Capture)

GPR56 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84915-1-PBS
Clone No.242249A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

ADGRG1 N-terminal fragment, ADGRG1 NT, ADGRG1 C-terminal fragment, ADGRG1 CT, ADGRG1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84915-1-PBS targets GPR56 as part of a matched antibody pair:

MP01673-1: 84915-1-PBS capture and 84915-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01673-2: 84915-1-PBS capture and 84915-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 56
Calculated molecular weight78 kDa
Observed molecular weight65 kDa
GenBank accession numberNM_001145771.2
Gene Symbol GPR56
Gene ID (NCBI) 9289
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y653
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GPR56 (G-protein-coupled receptor 56, also named ADGRG1) is a member of the adhesion G-protein coupled receptor (aGPCR) family and one of the important players in the normal development of the brain. It plays a pivotal role in diverse neurobiological processes, including cortical formation, oligodendrocyte development, and myelination (PMID: 32798453). Moreover, GPR56 has been reported to regulate VEGF secretion and angiogenesis in melanoma (PMID: 37579299).