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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPR65 Polyclonal antibody

GPR65 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20306-1-AP

Synonyms

G protein coupled receptor 65, GPR65, hTDAG8, Psychosine receptor, TDAG8

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using GPR65 Polyclonal antibody (20306-1-AP)

    IHC staining of human tonsillitis using 20306-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20306-1-AP (GPR65 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using GPR65 Polyclonal antibody (20306-1-AP)

    IHC staining of human tonsillitis using 20306-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20306-1-AP (GPR65 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20306-1-AP targets GPR65 in IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR65 fusion protein Ag14139 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 65
Calculated molecular weight 337 aa, 39 kDa
GenBank accession numberBC035633
Gene symbol GPR65
Gene ID (NCBI) 8477
RRIDAB_2878668
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPR65 (G protein-coupled receptor 65) was defined originally as T-cell death-associated gene 8 (TDAG8), functions as a proton-sensing receptor, and is also sensitive to psychosine (PMID: 35343079). GPR65 promotes adaptation to an acidic environment to enhance cell survival and proliferation, thereby promoting tumor development (PMID: 36852075).

Protocols

Product Specific Protocols
IHC protocol for GPR65 antibody 20306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Neurosurg Rev

GPR65 contributes to constructing immunosuppressive microenvironment in glioma

Authors - Jikang Fan
View Article