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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPR87 Polyclonal antibody

GPR87 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25999-1-AP

Synonyms

FKSG78, G protein coupled receptor 87, G protein coupled receptor 95, GPR87, GPR95, KPG_002

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse bladder tissue using GPR87 Polyclonal antibody (25999-1-AP)

    IHC staining of mouse bladder using 25999-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 25999-1-AP (GPR87 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using GPR87 Polyclonal antibody (25999-1-AP)

    IHC staining of mouse bladder using 25999-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 25999-1-AP (GPR87 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25999-1-AP targets GPR87 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR87 fusion protein Ag23214 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 87
GenBank accession numberBC132888
Gene symbol GPR87
Gene ID (NCBI) 53836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

G Protein-Coupled Receptor 87 (GPR87) is a GPCR, and it has been demonstrated to regulate the progression of several tumors (PMID:18183596). Studied reported that GPR87 promotes PDA (Pancreatic ductal adenocarcinoma) proliferation, angiogenesis, gemcitabine resistance, and tumorigenicity through activating the nuclear factor-kB (NF-kB) pathway (PMID:32405536). GPR87 is an independent prognostic factor for bladder cancer intravesical recurrence; it promotes bladder cancer cell proliferation and inhibits apoptosis. In addition, GPR87 was deorphanized and shown to be a lysophosphatidic acid (LPA) receptor (PMID:23752273).

Protocols

Product Specific Protocols
IHC protocol for GPR87 antibody 25999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols