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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GPRASP1-Specific Polyclonal antibody (20020-1-AP)

    WB analysis of HeLa using 20020-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using GPRASP1-Specific Polyclonal antibody (20020-1-AP)

    WB analysis of human brain using 20020-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GPRASP1-Specific Polyclonal antibody (20020-1-AP)

    WB analysis of HepG2 using 20020-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GPRASP1-Specific Polyclonal antibody (20020-1-AP)

    WB analysis of mouse brain using 20020-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using GPRASP1-Specific Polyclonal antibody (20020-1-AP)

    IHC staining of human brain using 20020-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using GPRASP1-Specific Polyclonal antibody (20020-1-AP)

    IHC staining of human brain using 20020-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20020-1-AP (GPRASP1-Specific antibody) at dilution of 1:100 (under 40x lens).

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GPRASP1-Specific Polyclonal antibody

GPRASP1-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20020-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Synonyms

GASP, GASP 1, GASP1, GPRASP1, GPRASP1-Specific, KIAA0443

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human brain tissue, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20020-1-AP targets GPRASP1-Specific in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor associated sorting protein 1
Calculated molecular weight 157 kDa
Observed molecular weight 157 kDa
GenBank accession numberNM_014710
Gene Symbol GPRASP1
Gene ID (NCBI) 9737
RRIDAB_10694144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JY77
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPRASP1, also named as GASP, GASP1 and KIAA0443, belongs to the GPRASP family. GPRASP1 may modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. It is targets receptors for degradation in lysosomes. The antibody is specific to GPRASP1.

Protocols

Product Specific Protocols
WB protocol for GPRASP1-Specific antibody 20020-1-APDownload protocol
IHC protocol for GPRASP1-Specific antibody 20020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Aging (Albany NY)

Sesamin-mediated high expression of BECN2 ameliorates cartilage endplate degeneration by reducing autophagy and inflammation

Authors - Baining Zhang
View Article