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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using GPRASP2 Polyclonal antibody (12159-1-AP)

    WB analysis of HEK-293 using 12159-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12159-1-AP (GPRASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GPRASP2 Polyclonal antibody (12159-1-AP)

    WB analysis of HeLa using 12159-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12159-1-AP (GPRASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GPRASP2 Polyclonal antibody (12159-1-AP)

    IHC staining of human gliomas using 12159-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12159-1-AP (GPRASP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GPRASP2 Polyclonal antibody (12159-1-AP)

    IHC staining of human gliomas using 12159-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12159-1-AP (GPRASP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

GPRASP2 Polyclonal antibody

GPRASP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12159-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

GASP 2, GASP2, GPRASP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12159-1-AP targets GPRASP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPRASP2 fusion protein Ag2800 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor associated sorting protein 2
Calculated molecular weight 838 aa, 94 kDa
Observed molecular weight 94 kDa
GenBank accession numberBC013576
Gene Symbol GPRASP2
Gene ID (NCBI) 114928
RRIDAB_2232653
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96D09
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GPRASP2 antibody 12159-1-APDownload protocol
IHC protocol for GPRASP2 antibody 12159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Abnormal mGluR-mediated synaptic plasticity and autism-like behaviours in Gprasp2 mutant mice.

Authors - Mohamed Edfawy
  • KO Validated
View Article
mouseIHC

J Med Genet

GPRASP2, a novel causative gene mutated in an X-linked recessive syndromic hearing loss.

Authors - Guangqian Xing
View Article