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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPRC5D Polyclonal antibody

GPRC5D Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21089-1-AP

Synonyms

G-protein coupled receptor family C group 5 member D

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GPRC5D Polyclonal antibody (21089-1-AP)

    WB analysis using 21089-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21089-1-AP (GPRC5D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GPRC5D Polyclonal antibody (21089-1-AP)

    IHC staining of human kidney using 21089-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21089-1-AP (GPRC5D Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GPRC5D Polyclonal antibody (21089-1-AP)

    IHC staining of human kidney using 21089-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21089-1-AP (GPRC5D Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inJurkat cells, THP-1 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21089-1-AP targets GPRC5D in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPRC5D fusion protein Ag15338 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor, family C, group 5, member D
Calculated molecular weight 345 aa, 39 kDa
Observed molecular weight34-39 kDa
GenBank accession numberBC069341
Gene symbol GPRC5D
Gene ID (NCBI) 55507
RRIDAB_2878810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GPRC5D antibody 21089-1-APDownload protocol
IHC protocol for GPRC5D antibody 21089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols