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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using GPRIN3-Specific Polyclonal antibody (20212-1-AP)

    WB analysis of human brain using 20212-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20212-1-AP (GPRIN3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GPRIN3-Specific Polyclonal antibody (20212-1-AP)

    WB analysis of mouse brain using 20212-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20212-1-AP (GPRIN3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using GPRIN3-Specific Polyclonal antibody (20212-1-AP)

    IHC staining of human stomach using 20212-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20212-1-AP (GPRIN3-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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GPRIN3-Specific Polyclonal antibody

GPRIN3-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20212-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

GPRIN3, KIAA2027, GRIN3, GPRIN family member 3, G protein-regulated inducer of neurite outgrowth 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20212-1-AP targets GPRIN3-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name GPRIN family member 3
Calculated molecular weight 82 kDa
Observed molecular weight 82 kDa
GenBank accession numberNM_198281
Gene Symbol GPRIN3
Gene ID (NCBI) 285513
RRIDAB_10666200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZVF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPRIN3, also named as KIAA2027, may be involved in neurite outgrowth. The antibody is specific to GPRIN3.

Protocols

Product Specific Protocols
WB protocol for GPRIN3-Specific antibody 20212-1-APDownload protocol
IHC protocol for GPRIN3-Specific antibody 20212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell Biol

UBE3D Regulates mRNA 3\'-End Processing and Maintains Adipogenic Potential in 3T3-L1 Cells

Authors - Daniel Heller-Trulli
View Article