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  • Featured Product
  • KD/KO Validated

GPX3 Polyclonal antibody, PBS Only

GPX3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13947-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using GPX3 Polyclonal antibody (13947-1-AP)

    WB analysis of mouse kidney using 13947-1-AP (same clone as 13947-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13947-1-AP (GPX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat eye tissue using GPX3 Polyclonal antibody (13947-1-AP)

    IHC staining of rat eye using 13947-1-AP (same clone as 13947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat eye tissue using GPX3 Polyclonal antibody (13947-1-AP)

    IHC staining of rat eye using 13947-1-AP (same clone as 13947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GPX3 Polyclonal antibody (13947-1-AP)

    IHC staining of mouse kidney using 13947-1-AP (same clone as 13947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GPX3 Polyclonal antibody (13947-1-AP)

    IHC staining of mouse kidney using 13947-1-AP (same clone as 13947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13947-1-AP (GPX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13947-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using GPX3 Polyclonal antibody (13947-1-AP)

    IF Staining of HEK-293 using 13947-1-AP (same clone as 13947-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GPX3 antibody (13947-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13947-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13947-1-PBS targets GPX3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPX3 fusion protein Ag5054 相同性解析による交差性が予測される生物種
Full Name glutathione peroxidase 3 (plasma)
Calculated molecular weight 226 aa, 26 kDa
Observed molecular weight 20-27 kDa
GenBank accession numberBC050378
Gene symbol GPX3
Gene ID (NCBI) 2878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GPX3 (glutathione peroxidase 3) is also named as Plasma Glutathione Peroxidase. GPX3 can protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPX3 is secreted into plasma and like GPX1, is an 100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. It can be detected a band of 20 kDa which is considered as the chain A of GPX3 (selenocysteine to glycine mutant).