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- KD/KO Validated
GPX4 Polyclonal antibody
GPX4 Polyclonal Antibody for WB, IF-P, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF-P, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 30388-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, HL-60 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | rat testis tissue |
| Positive IF-P detected in | rat testis tissue |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 58 publications below |
| IHC | See 12 publications below |
| IF | See 9 publications below |
| FC | See 1 publications below |
Product Information
30388-1-AP targets GPX4 in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GPX4 fusion protein Ag30650 相同性解析による交差性が予測される生物種 |
| Full Name | glutathione peroxidase 4 (phospholipid hydroperoxidase) |
| Observed molecular weight | 18-23 kDa |
| GenBank accession number | BC021567 |
| Gene symbol | GPX4 |
| Gene ID (NCBI) | 2879 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPX4 antibody 30388-1-AP | Download protocol |
| IF protocol for GPX4 antibody 30388-1-AP | Download protocol |
| IP protocol for GPX4 antibody 30388-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater A two-pronged approach to inhibit ferroptosis of MSCs caused by the iron overload in postmenopausal osteoporosis and promote osseointegration of titanium implant | ||
Sci Adv In situ implantable DNA hydrogel for diagnosis and therapy of postoperative rehemorrhage following intracerebral hemorrhage surgery | ||
Adv Healthc Mater Fe3 O4 -Incorporated Metal-Organic Framework for Chemo/Ferroptosis Synergistic Anti-Tumor via the Enhanced Chemodynamic Therapy | ||
Biomed Pharmacother AP39 inhibits ferroptosis by inhibiting mitochondrial autophagy through the PINK1/parkin pathway to improve myocardial fibrosis with myocardial infarction | ||
Int Immunopharmacol 4-Octyl itaconate protects chondrocytes against IL-1β-induced oxidative stress and ferroptosis by inhibiting GPX4 methylation in osteoarthritis | ||
J Ethnopharmacol Ginsenoside Rg1 alleviates chronic inflammation-induced neuronal ferroptosis and cognitive impairments via regulation of AIM2 - Nrf2 signaling pathway |
