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  • Featured Product
  • KD/KO Validated

GPX4 Monoclonal antibody, PBS Only

GPX4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F5G5

Cat no : 67763-1-PBS

Synonyms

Glutathione peroxidase 4, GPx 4, GPX4, GSHPx 4, MCSP, PHGPx, snGPx, snPHGPx

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPX4 Monoclonal antibody (67763-1-Ig)

    WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPX4 Monoclonal antibody (67763-1-Ig)

    WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPX4 Monoclonal antibody (67763-1-Ig)

    WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPX4 Monoclonal antibody (67763-1-Ig)

    WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPX4 Monoclonal antibody (67763-1-Ig)

    WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of human liver cancer using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of human liver cancer using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide not using 67763-1-Ig (GPX4 antibody) as negtive control. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide not using 67763-1-Ig (GPX4 antibody) as negtive control. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GPX4 Monoclonal antibody (67763-1-Ig)

    IF Staining of HeLa using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67763-1-PBS targets GPX4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GPX4 fusion protein Ag30650 相同性解析による交差性が予測される生物種
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Observed molecular weight 20-23 kDa
GenBank accession numberBC021567
Gene symbol GPX4
Gene ID (NCBI) 2879
RRIDAB_2909469
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.