• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GPX4 Monoclonal antibody (67763-1-Ig)

    WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPX4 Monoclonal antibody (67763-1-Ig)

    WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPX4 Monoclonal antibody (67763-1-Ig)

    WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GPX4 Monoclonal antibody (67763-1-Ig)

    WB analysis of HEK-293 using 67763-1-Ig (same clone as 67763-1-PBS)

    WB result of GPX4 antibody (67763-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 transfected HEK-293 cells. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPX4 Monoclonal antibody (67763-1-Ig)

    WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPX4 Monoclonal antibody (67763-1-Ig)

    WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of human liver cancer using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of human liver cancer using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide not using 67763-1-Ig (GPX4 antibody) as negtive control. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of rat testis using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide not using 67763-1-Ig (GPX4 antibody) as negtive control. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IHC staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using GPX4 Monoclonal antibody (67763-1-Ig)

    IF Staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GPX4 Monoclonal antibody (67763-1-Ig)

    IF Staining of HeLa using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

GPX4 Monoclonal antibody, PBS Only

GPX4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 67763-1-PBS
Clone No.3F5G5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, dog

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

3F5G5, EC:1.11.1.12, EC:1.11.1.9, GPx 4, GPx-4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67763-1-PBS targets GPX4 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, dog samples.

Tested Reactivity human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, dog
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GPX4 fusion protein Ag30650 相同性解析による交差性が予測される生物種
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Observed molecular weight 20-23 kDa
GenBank accession numberBC021567
Gene Symbol GPX4
Gene ID (NCBI) 2879
RRIDAB_2909469
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36969
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.