GPX5 Polyclonal antibody

GPX5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18731-1-AP

Synonyms

EGLP, Glutathione peroxidase 5, GPx 5, GPX5, GSHPx 5


☆大好評トライアルサイズ ¥20,000~! >>> 一次抗体特別価格キャンペーン開催中!


Tested Applications

Positive WB detected inmouse testis tissue, PC-3 cells, human testis tissue, mouse liver tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18731-1-AP targets GPX5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name glutathione peroxidase 5 (epididymal androgen-related protein)
Calculated molecular weight 25 kDa
Observed molecular weight 22-28 kDa
GenBank accession numberNM_001509
Gene symbol GPX5
Gene ID (NCBI) 2880
RRIDAB_10596913
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPX5 belongs to the glutathione peroxidase family. GPX5 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Catalytic the reaction: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. GPX5 constitute a glutathionine peroxidase-like protective system against peroxide damage in sperm membrane lipids. GPX5 has a calculated molecular mass of 25 kDa and apparent molecular mass of 22-28 kDa due to the cleavage of signal peptide and glycosylation.

Protocols

Product Specific Protocols
WB protocol for GPX5 antibody 18731-1-APDownload protocol
IHC protocol for GPX5 antibody 18731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
goatWB

Oxid Med Cell Longev

Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway.

Authors - Tian-Yu Feng
mouseIF

Sci Rep

Proteasome activators, PA28γ and PA200, play indispensable roles in male fertility.

Authors - Lin Huang
mouseWB

Mol Biol Rep

Autophagy-related 7 proteindependent autophagy mediates resveratrol-caused upregulation of mitochondrial biogenesis and steroidogenesis in aged Leydig cell

Authors - Fanhong Lin
pigWB

Animals (Basel)

Effect of Boar Sperm Proteins and Quality Changes on Field Fertility.

Authors - Ilias Michos
pigWB

Theriogenology

Effects of kojic acid on boar sperm quality and anti-bacterial activity during liquid preservation at 17 C.

Authors - Weike Shaoyong
humanWB

Anim Reprod Sci

Triosephosphate isomerase (TPI) and epididymal secretory glutathione peroxidase (GPX5) are markers for boar sperm quality.

Authors - Ingrid Vilagran