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KD/KO Validated

GPX7 Polyclonal antibody

Name: GPX7 Polyclonal antibody
Brand: Proteintech
SKU: 13501-1-AP

GPX7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13501-1-AP

Synonyms

CL683, glutathione peroxidase 7, GPx 7, GPX6, GPX7, GSHPx 7

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of human liver using 13501-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 13501-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13501-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Transfected HEK-293 using 13501-1-AP
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13501-1-AP (GPX7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 13501-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13501-1-AP (GPX7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13501-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13501-1-AP (GPX7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 13501-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 13501-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 13501-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 13501-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13501-1-AP (GPX7 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 13501-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human liver tissue, human placenta tissue, human brain tissue, Transfected HEK-293 cells
Positive IHC detected in	human liver cancer tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 12 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

13501-1-AP targets GPX7 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, chicken, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GPX7 fusion protein Ag4328 相同性解析による交差性が予測される生物種
Full Name	glutathione peroxidase 7
Calculated molecular weight	187 aa, 21 kDa
Observed molecular weight	18-21 kDa
GenBank accession number	BC032788
Gene symbol	GPX7
Gene ID (NCBI)	2882
RRID	AB_2112560
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GPX7(Glutathione peroxidase 7) is also named as GPX6,CL683,GSHPx-7 and belongs to the glutathione peroxidase family.It shares approximately 45% amino acid sequence with other GPX family members.Because GPX7 expression protected cells from acidic bile acidinduced DNA damage, it may protect oesophageal epithelial cells from acidic bile acid-induced apoptosis(PMID:22157330). This antibody can ve detected as 18-21kDa due to signal peptide splicing.

Protocols

Product Specific Protocols
WB protocol for GPX7 antibody 13501-1-AP	Download protocol
IHC protocol for GPX7 antibody 13501-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Gut Glutathione peroxidase 7 protects against oxidative DNA damage in oesophageal cells. Authors - Peng Dunfa D View Article
human	WB	Gut Glutathione peroxidase 7 has potential tumour suppressor functions that are silenced by location-specific methylation in oesophageal adenocarcinoma. Authors - Peng Dunfa D View Article
mouse	WB	EMBO Mol Med Deficiency of NPGPx, an oxidative stress sensor, leads to obesity in mice and human. Authors - Chang Yi-Cheng YC View Article
human	IHC	Oncogene Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling. Authors - Qingcai Meng View Article
human	WB	Carcinogenesis Loss of glutathione peroxidase 7 promotes TNF-α-induced NF-κB activation in Barrett's carcinogenesi. Authors - Dun-Fa Peng KD Validated View Article
	WB	Free Radic Biol Med GPx8 peroxidase prevents leakage of H2O2 from the endoplasmic reticulum. Authors - Thomas Ramming View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPX7 Polyclonal antibody

GPX7 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of human liver using 13501-1-AP

WB analysis using 13501-1-AP

WB analysis of human brain using 13501-1-AP

WB analysis of Transfected HEK-293 using 13501-1-AP

IHC staining of human liver cancer using 13501-1-AP

IHC staining of human liver cancer using 13501-1-AP

IHC staining of human brain using 13501-1-AP

IHC staining of human brain using 13501-1-AP

IHC staining of human brain using 13501-1-AP

IHC staining of human brain using 13501-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Glutathione peroxidase 7 protects against oxidative DNA damage in oesophageal cells.

Glutathione peroxidase 7 has potential tumour suppressor functions that are silenced by location-specific methylation in oesophageal adenocarcinoma.

Deficiency of NPGPx, an oxidative stress sensor, leads to obesity in mice and human.

Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.

Loss of glutathione peroxidase 7 promotes TNF-α-induced NF-κB activation in Barrett's carcinogenesi.

GPx8 peroxidase prevents leakage of H2O2 from the endoplasmic reticulum.