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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using GRAMD1B Polyclonal antibody (24905-1-AP)

    WB analysis of A549 using 24905-1-AP (same clone as 24905-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 24905-1-AP (GRAMD1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24905-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GRAMD1B Polyclonal antibody (24905-1-AP)

    IHC staining of human liver cancer using 24905-1-AP (same clone as 24905-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24905-1-AP (GRAMD1B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24905-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GRAMD1B Polyclonal antibody (24905-1-AP)

    IHC staining of human liver cancer using 24905-1-AP (same clone as 24905-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24905-1-AP (GRAMD1B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24905-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using GRAMD1B Polyclonal antibody (24905-1-AP)

    IF Staining of A549 using 24905-1-AP (same clone as 24905-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 24905-1-AP (GRAMD1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24905-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using GRAMD1B Polyclonal antibody (24905-1-AP)

    FC experiment of A549 using 24905-1-AP (same clone as 24905-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human GRAMD1B (24905-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 24905-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GRAMD1B Polyclonal antibody, PBS Only

GRAMD1B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 24905-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24905-1-PBS targets GRAMD1B in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRAMD1B fusion protein Ag20670 相同性解析による交差性が予測される生物種
Full Name GRAM domain containing 1B
Calculated molecular weight 738 aa, 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC107480
Gene Symbol GRAMD1B
Gene ID (NCBI) 57476
RRIDAB_2879791
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3KR37
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GRAMD1B, also named as KIAA1201, contains a transmembrane region and two domains of known function; the GRAM domain and a VASt domain. It is predicted to localize in the nucleus, supported by several nuclear transport signals and nuclearly associated motifs. This highly conserved gene is found in a variety of vertebrates and invertebrates, however, it is not found in bacteria or fungi. There're some isoforms with MW 50 kDa and 81-87 kDa. With phosphor modification, it's about 90-110 kDa in WB detection.