GRASP65,GORASP1 Polyclonal antibody
GRASP65,GORASP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 10747-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IF | See 1 publications below |
Product Information
10747-2-AP targets GRASP65,GORASP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GRASP65,GORASP1 fusion protein Ag1054 相同性解析による交差性が予測される生物種 |
| Full Name | golgi reassembly stacking protein 1, 65kDa |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC008928 |
| Gene symbol | GRASP65 |
| Gene ID (NCBI) | 64689 |
| RRID | AB_2113204 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. (refer to Wikipedia)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRASP65,GORASP1 antibody 10747-2-AP | Download protocol |
| IF protocol for GRASP65,GORASP1 antibody 10747-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience Cytosolic Ca2+ Modulates Golgi Structure Through PKCα-Mediated GRASP55 Phosphorylation. | ||
J Cell Biochem Golgi apparatus fragmentation participates in oxidized low-density lipoprotein-induced endothelial cell injury. | ||
Biochem Biophys Res Commun Golgi damage caused by dysfunction of PiT-2 in primary familial brain calcification | ||
Cell Death Dis GOLPH3 promotes endotoxemia-induced liver and kidney injury through Golgi stress-mediated apoptosis and inflammatory response | ||
Prostaglandins Other Lipid Mediat GOLPH3 knockdown alleviates the inflammation and apoptosis in lipopolysaccharide-induced acute lung injury by inhibiting Golgi stress mediated autophagy |
