Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HSC-T6 cells, HEK-293 cells, MCF-7 cells, Jurkat cells |
| Positive IHC detected in | human prostate cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SK-BR-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1938-1:7752 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
66651-1-Ig targets GRASP65,GORASP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GRASP65,GORASP1 fusion protein Ag1054 相同性解析による交差性が予測される生物種 |
| Full Name | golgi reassembly stacking protein 1, 65kDa |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC008928 |
| Gene Symbol | GRASP65 |
| Gene ID (NCBI) | 64689 |
| RRID | AB_2882008 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BQQ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRASP65,GORASP1 antibody 66651-1-Ig | Download protocol |
| IHC protocol for GRASP65,GORASP1 antibody 66651-1-Ig | Download protocol |
| IF protocol for GRASP65,GORASP1 antibody 66651-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration. | ||
Mol Biol Cell Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking. | ||
J Cell Biochem Golgi apparatus fragmentation participates in oxidized low-density lipoprotein-induced endothelial cell injury. | ||
Cells Discovery of Cell Number-Interstitial Fluid Volume (CIF) Ratio Reveals Secretory Autophagy Pathway to Supply eHsp90α for Wound Healing
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