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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRASP65,GORASP1 Polyclonal antibody, PBS Only

GRASP65,GORASP1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10747-2-PBS

Synonyms

GORASP1, GRASP65, GOLPH5, Golgi reassembly-stacking protein of 65 kDa, Golgi reassembly-stacking protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRASP65,GORASP1 Polyclonal antibody (10747-2-AP)

    WB analysis using 10747-2-AP (same clone as 10747-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10747-2-AP (GRASP65,GORASP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10747-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GRASP65,GORASP1 Polyclonal antibody (10747-2-AP)

    IF Staining of HeLa using 10747-2-AP (same clone as 10747-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GRASP65,GORASP1 antibody (10747-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10747-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GRASP65,GORASP1 Polyclonal antibody (10747-2-AP)

    IF Staining of HeLa using 10747-2-AP (same clone as 10747-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GRASP65,GORASP1 antibody (10747-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 10747-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10747-2-PBS targets GRASP65,GORASP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRASP65,GORASP1 fusion protein Ag1054 相同性解析による交差性が予測される生物種
Full Name golgi reassembly stacking protein 1, 65kDa
Calculated molecular weight 46 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC008928
Gene symbol GRASP65
Gene ID (NCBI) 64689
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. (refer to Wikipedia)