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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRB10 Polyclonal antibody, PBS Only

GRB10 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23591-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRB10 Polyclonal antibody (23591-1-AP)

    WB analysis using 23591-1-AP (same clone as 23591-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23591-1-AP (GRB10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23591-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GRB10 Polyclonal antibody (23591-1-AP)

    WB analysis of HepG2 using 23591-1-AP (same clone as 23591-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23591-1-AP (GRB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23591-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GRB10 Polyclonal antibody (23591-1-AP)

    IHC staining of human liver using 23591-1-AP (same clone as 23591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23591-1-AP (GRB10 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23591-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GRB10 Polyclonal antibody (23591-1-AP)

    IHC staining of human liver using 23591-1-AP (same clone as 23591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23591-1-AP (GRB10 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23591-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GRB10 Polyclonal antibody (23591-1-AP)

    IHC staining of human kidney using 23591-1-AP (same clone as 23591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23591-1-AP (GRB10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23591-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GRB10 Polyclonal antibody (23591-1-AP)

    IHC staining of human kidney using 23591-1-AP (same clone as 23591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23591-1-AP (GRB10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23591-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GRB10 Polyclonal antibody (23591-1-AP)

    IF Staining of HepG2 using 23591-1-AP (same clone as 23591-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRB10 antibody (23591-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23591-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23591-1-PBS targets GRB10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRB10 fusion protein Ag20355 相同性解析による交差性が予測される生物種
Full Name growth factor receptor-bound protein 10
Calculated molecular weight536aa,61 kDa; 594aa,67 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC024285
Gene symbol GRB10
Gene ID (NCBI) 2887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GRB10 is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including INSR and IGF1R. It may play a role in mediating ubiquitination of INSR, leading to proteasomal degradation.