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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRB10 Polyclonal antibody

GRB10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28872-1-AP

Synonyms

Grb 10, GRB IR, GRB10, GRB10 adapter protein, GRBIR, IRBP, KIAA0207, MEG1, RSS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRB10 Polyclonal antibody (28872-1-AP)

    WB analysis using 28872-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28872-1-AP (GRB10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using GRB10 Polyclonal antibody (28872-1-AP)

    IHC staining of human liver using 28872-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28872-1-AP (GRB10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using GRB10 Polyclonal antibody (28872-1-AP)

    IHC staining of human liver using 28872-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28872-1-AP (GRB10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inBxPC-3 cells, HepG2 cells, mouse brain tissue, mouse pancreas tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28872-1-AP targets GRB10 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name growth factor receptor-bound protein 10
Calculated molecular weight536aa,61 kDa; 594aa,67 kDa
Observed molecular weight60-70 kDa
GenBank accession numberBC024285
Gene symbol GRB10
Gene ID (NCBI) 2887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRB10 is an adapter protein that modulates the coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain, and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including INSR and IGF1R. It may play a role in mediating ubiquitination of INSR, leading to proteasomal degradation. GRB10 has 4 isoforms with the molecular mass of 60-70 kDa, which are produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for GRB10 antibody 28872-1-APDownload protocol
IHC protocol for GRB10 antibody 28872-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols