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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRB10 Recombinant antibody (81225-1-RR)

    WB analysis using 81225-1-RR (same clone as 81225-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81225-1-RR (GRB10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81225-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using GRB10 Recombinant antibody (81225-1-RR)

    IF Staining of LNCaP using 81225-1-RR (same clone as 81225-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using GRB10 antibody (81225-1-RR, Clone: 1D13 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81225-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81225-1-RR

    Affinity and Kinetic Characterization of 81225-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81225-1-RR against Human GRB10 were performed. The affinity constant is 0.195 nM.

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GRB10 Recombinant antibody, PBS Only

GRB10 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 81225-1-PBS
Clone No.1D13

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KIAA0207, Insulin receptor-binding protein Grb-IR, Growth factor receptor-bound protein 10, GRBIR, 1D13

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81225-1-PBS targets GRB10 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GRB10 fusion protein Ag20355 相同性解析による交差性が予測される生物種
Full Name growth factor receptor-bound protein 10
Calculated molecular weight536aa,61 kDa; 594aa,67 kDa
Observed molecular weight60-70 kDa
GenBank accession numberBC024285
Gene Symbol GRB10
Gene ID (NCBI) 2887
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13322
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GRB10 is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including INSR and IGF1R. It may play a role in mediating ubiquitination of INSR, leading to proteasomal degradation. GRB10 has 4 isoforms with the molecular mass of 60-70 kDa, which are produced by alternative splicing.