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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRB14 Polyclonal antibody

GRB14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15298-1-AP

Synonyms

GRB14, GRB14 adapter protein

Validation Data Gallery

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  • Western Blot (WB) analysis of DU 145 cells using GRB14 Polyclonal antibody (15298-1-AP)

    WB analysis of DU 145 using 15298-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 15298-1-AP (GRB14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using GRB14 Polyclonal antibody (15298-1-AP)

    IHC staining of human ovary tumor using 15298-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15298-1-AP (GRB14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using GRB14 Polyclonal antibody (15298-1-AP)

    IHC staining of human ovary tumor using 15298-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15298-1-AP (GRB14 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inDU 145 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15298-1-AP targets GRB14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRB14 fusion protein Ag7554 相同性解析による交差性が予測される生物種
Full Name growth factor receptor-bound protein 14
Calculated molecular weight 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC053559
Gene symbol GRB14
Gene ID (NCBI) 2888
RRIDAB_2112892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRB14 belongs to the GRB7/10/14 family. It is an adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. GRB14 is a target for a PDGF regulated serine kinase, an interaction that does not require PDGFR-GRB14 association. GRB14 is about 58-65kd in WB test.

Protocols

Product Specific Protocols
WB protocol for GRB14 antibody 15298-1-APDownload protocol
IHC protocol for GRB14 antibody 15298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols