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GRB2 Polyclonal antibody

Name: GRB2 Polyclonal antibody
Brand: Proteintech
SKU: 10254-2-AP

GRB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10254-2-AP

Synonyms

Growth factor receptor-bound protein 2, Grb33, EGFRBP GRB2, ASH

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of HEK-293 using 10254-2-AP
WB result of GRB2 antibody (10254-2-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRB2 transfected HEK-293 cells.
WB analysis of HEK-293 using 10254-2-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10254-2-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 10254-2-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
WB analysis of rat spleen using 10254-2-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 10254-2-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10254-2-AP (GRB2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 10254-2-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10254-2-AP (GRB2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 10254-2-AP
Immunofluorescent analysis of HEK-293 cells using 10254-2-AP (GRB2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10254-2-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, A431 cells, rat brain tissue, rat spleen tissue
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 12 publications below
IHC	See 4 publications below
IF	See 2 publications below

Product Information

10254-2-AP targets GRB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GRB2 fusion protein Ag0395 相同性解析による交差性が予測される生物種
Full Name	growth factor receptor-bound protein 2
Calculated molecular weight	25 kDa
Observed molecular weight	25-28 kDa
GenBank accession number	BC000631
Gene symbol	GRB2
Gene ID (NCBI)	2885
RRID	AB_2263577
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-beta protein precursor. Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.

Protocols

Product Specific Protocols
WB protocol for GRB2 antibody 10254-2-AP	Download protocol
IHC protocol for GRB2 antibody 10254-2-AP	Download protocol
IF protocol for GRB2 antibody 10254-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC,WB	Mol Ther Nucleic Acids ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway. Authors - Lingjiao Meng View Article
human	WB	Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. Authors - Hongwei Chu View Article
human	IHC	Cancer Lett Deubiquitinase PSMD14 enhances hepatocellular carcinoma growth and metastasis by stabilizing GRB2. Authors - Jie Lv View Article
human	WB,IHC	Oncotarget mir-329 restricts tumor growth by targeting grb2 in pancreatic cancer. Authors - Xinjing Wang View Article
human	WB	Food Funct Extraction, purification and anticancer activity studies on triterpenes from pomegranate peel Authors - Mengqi Zhang View Article
		Cancer Lett Melittin inhibits tumor growth and decreases resistance to gemcitabine by downregulating cholesterol pathway gene CLU in pancreatic ductal adenocarcinoma. Authors - Xinjing Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRB2 Polyclonal antibody

GRB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 10254-2-AP

WB analysis of HEK-293 using 10254-2-AP

WB analysis of A431 using 10254-2-AP

WB analysis of rat brain using 10254-2-AP

WB analysis of rat spleen using 10254-2-AP

IHC staining of human pancreas cancer using 10254-2-AP

IHC staining of human pancreas cancer using 10254-2-AP

IF Staining of HEK-293 using 10254-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway.

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Deubiquitinase PSMD14 enhances hepatocellular carcinoma growth and metastasis by stabilizing GRB2.

mir-329 restricts tumor growth by targeting grb2 in pancreatic cancer.

Extraction, purification and anticancer activity studies on triterpenes from pomegranate peel

Melittin inhibits tumor growth and decreases resistance to gemcitabine by downregulating cholesterol pathway gene CLU in pancreatic ductal adenocarcinoma.