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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GREB1 Polyclonal antibody

GREB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28699-1-AP

Synonyms

GREB1, GREB1 protein, KIAA0575, Protein GREB1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GREB1 Polyclonal antibody (28699-1-AP)

    WB analysis using 28699-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28699-1-AP (GREB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using GREB1 Polyclonal antibody (28699-1-AP)

    WB analysis of MCF-7 using 28699-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 28699-1-AP (GREB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using GREB1 Polyclonal antibody (28699-1-AP)

    IHC staining of human liver using 28699-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28699-1-AP (GREB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using GREB1 Polyclonal antibody (28699-1-AP)

    IHC staining of human liver using 28699-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28699-1-AP (GREB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GREB1 Polyclonal antibody (28699-1-AP)

    IF Staining of HepG2 using 28699-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GREB1 antibody (28699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GREB1 Polyclonal antibody (28699-1-AP)

    IF Staining of HepG2 using 28699-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GREB1 antibody (28699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28699-1-AP targets GREB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GREB1 fusion protein Ag29908 相同性解析による交差性が予測される生物種
Full Name GREB1 protein
Calculated molecular weight 216 kDa
Observed molecular weight 216-240 kDa
GenBank accession numberNM_014668
Gene symbol GREB1
Gene ID (NCBI) 9687
RRIDAB_2881196
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GREB1 (Gene regulated by estrogen in breast cancer protein1) encodes a 1949-amino acid protein that contains an N-terminal Zn-binding domain followed by a circularly permuted catalytically inactive superfamily II helicase domain and a C-terminal Fringe-like GT domain. GREB1 is an early estrogen-responsive gene, and its expression is correlated with estrogen levels in breast cancer patients. GREB1 is implicated in hormone-dependent cancers, such as ovarian, prostate cancer and as a risk factor in endometriosis and variations in bone mineral density.

Protocols

Product Specific Protocols
WB protocol for GREB1 antibody 28699-1-APDownload protocol
IHC protocol for GREB1 antibody 28699-1-APDownload protocol
IF protocol for GREB1 antibody 28699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cancer Gene Ther

Tafazzin mediates tamoxifen resistance by regulating cellular phospholipid composition in ER-positive breast cancer

Authors - Xuan Li
View Article
humanWB

Cancers (Basel)

Pharmacological Inhibition of CBP/p300 Blocks Estrogen Receptor Alpha (ERα) Function through Suppressing Enhancer H3K27 Acetylation in Luminal Breast Cancer.

Authors - Aaron Waddell
View Article