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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRIA1 Polyclonal antibody

GRIA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Rat, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25012-1-AP

Synonyms

GluA1, GLUH1, GluR 1, GluR A, GluR K1, GLUR1, GLURA, Glutamate receptor 1, GRIA1, HBGR1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRIA1 Polyclonal antibody (25012-1-AP)

    WB analysis using 25012-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25012-1-AP (GRIA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GRIA1 Polyclonal antibody (25012-1-AP)

    WB analysis using 25012-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25012-1-AP (GRIA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using GRIA1 Polyclonal antibody (25012-1-AP)

    IHC staining of human brain using 25012-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25012-1-AP (GRIA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using GRIA1 Polyclonal antibody (25012-1-AP)

    IHC staining of human brain using 25012-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25012-1-AP (GRIA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GRIA1 Polyclonal antibody (25012-1-AP)

    IHC staining of mouse brain using 25012-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25012-1-AP (GRIA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25012-1-AP targets GRIA1 in WB, IHC, ELISA applications and shows reactivity with human, Rat, Mouse samples.

Tested Reactivity human, Rat, Mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRIA1 fusion protein Ag21502 相同性解析による交差性が予測される生物種
Full Name glutamate receptor, ionotropic, AMPA 1
Calculated molecular weight 906 aa, 102 kDa
Observed molecular weight100 kDa
GenBank accession numberBC111734
Gene symbol GRIA1
Gene ID (NCBI) 2890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors.

Protocols

Product Specific Protocols
WB protocol for GRIA1 antibody 25012-1-APDownload protocol
IHC protocol for GRIA1 antibody 25012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Ethnopharmacol

Iridoids from Gardeniae fructus ameliorates depression by enhancing synaptic plasticity via AMPA receptor-mTOR signaling

Authors - Baomei Xia
View Article