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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Glutamate receptor 3/GluA3 Polyclonal antibody

Glutamate receptor 3/GluA3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 29588-1-AP

Synonyms

GRIA3, GLURC, GLUR3, GLUR K3, GLUR C

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GRIA3 Polyclonal antibody (29588-1-AP)

    WB analysis using 29588-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29588-1-AP (GRIA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Glutamate receptor 3/GluA3 Polyclonal antibody (29588-1-AP)

    WB analysis using 29588-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29588-1-AP (Glutamate receptor 3/GluA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GRIA3 Polyclonal antibody (29588-1-AP)

    IP experiment of mouse brain using 29588-1-AP

    IP result of anti-GRIA3 (IP:29588-1-AP, 4ug; Detection:29588-1-AP 1:1000) with mouse brain tissue lysate 1280 ug.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Glutamate receptor 3/GluA3 Polyclonal antibody (29588-1-AP)

    IP experiment of mouse brain using 29588-1-AP

    IP result of anti-Glutamate receptor 3/GluA3 (IP:29588-1-AP, 4ug; Detection:29588-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

29588-1-AP targets Glutamate receptor 3/GluA3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glutamate receptor 3/GluA3 fusion protein Ag30898 相同性解析による交差性が予測される生物種
Full Name glutamate receptor, ionotrophic, AMPA 3
Calculated molecular weight 101 kDa
Observed molecular weight101 kDa
GenBank accession numberNM_007325
Gene symbol GRIA3
Gene ID (NCBI) 2892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA3 (Glutamate ionotropic receptor AMPA type subunit 3), also known as Glutamate receptor R3 or A3 (GluR3 or GluA3), is associated with X-linked intellectual disability (ID), dysmorphic features, and non-syndromic epilepsy(PMID: 32977175). GRIA3 also plays a role as a mediator of tumor progression in pancreatic cancer downstream CUX1(PMID: 20689760).

Protocols

Product Specific Protocols
WB protocol for Glutamate receptor 3/GluA3 antibody 29588-1-APDownload protocol
IP protocol for Glutamate receptor 3/GluA3 antibody 29588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

iScience

The decreased astrocyte-microglia interaction reflects the early characteristics of Alzheimer's disease

Authors - Kefu Liu
View Article