GRIA4 Polyclonal antibody
GRIA4 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 23350-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat brain tissue, mouse brain tissue, mouse cerebellum tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IHC | See 2 publications below |
| IP | See 1 publications below |
Product Information
The immunogen of 23350-1-AP is GRIA4 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GRIA4 fusion protein Ag19949 相同性解析による交差性が予測される生物種 |
| Full Name | glutamate receptor, ionotrophic, AMPA 4 |
| Calculated molecular weight | 902 aa, 101 kDa |
| Observed molecular weight | 101 kDa |
| GenBank accession number | BC167786 |
| Gene symbol | GRIA4 |
| Gene ID (NCBI) | 2893 |
| RRID | AB_2879262 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRIA4 antibody 23350-1-AP | Download protocol |
| IHC protocol for GRIA4 antibody 23350-1-AP | Download protocol |
| IP protocol for GRIA4 antibody 23350-1-AP | Download protocol |
| FC protocol for GRIA4 antibody 23350-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Psychiatry A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling. | ||
J Clin Invest TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation. | ||
Front Cell Dev Biol Olfactomedin-3 Enhances Seizure Activity by Interacting With AMPA Receptors in Epilepsy Models. | ||
Mol Brain Assessments of prolonged effects of desflurane and sevoflurane on motor learning deficits in aged AppNL-G-F/NL-G-F mice. | ||
J Psychiatr Res Hippocampal AMPA receptors mediate the impairment of spatial learning and memory in prenatally stressed offspring rats. |
