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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using GRID1 Polyclonal antibody (13040-1-AP)

    WB analysis of human brain using 13040-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13040-1-AP (GRID1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IP experiment of mouse brain using 13040-1-AP

    IP result of anti-GRID1 (IP:13040-1-AP, 4ug; Detection:13040-1-AP 1:300) with mouse brain tissue lysate 5600ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IHC staining of human gliomas using 13040-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IHC staining of human gliomas using 13040-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IHC staining of human brain using 13040-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IHC staining of human brain using 13040-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13040-1-AP (GRID1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IHC staining of mouse brain using 13040-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13040-1-AP (GRID1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GRID1 Polyclonal antibody (13040-1-AP)

    IHC staining of mouse brain using 13040-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13040-1-AP (GRID1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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GRID1 Polyclonal antibody

GRID1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13040-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

KIAA1220, Glutamate receptor ionotropic, delta-1, GluR delta-1 subunit, GluR delta 1 subunit, GluD1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

13040-1-AP targets GRID1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRID1 fusion protein Ag3694 相同性解析による交差性が予測される生物種
Full Name glutamate receptor, ionotropic, delta 1
Calculated molecular weight 1009 aa, 112 kDa
Observed molecular weight 100-112 kDa
GenBank accession numberBC039263
Gene Symbol GRID1
Gene ID (NCBI) 2894
RRIDAB_10638439
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULK0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRID1 (Glutamate Ionotropic Receptor Delta Type Subunit 1) is a protein-coding gene that belongs to the family of ionotropic glutamate receptors. These receptors are crucial for rapid excitatory synaptic transmission and synaptic plasticity in the central nervous system. GRID1 is primarily expressed in various brain regions, including the cortex, hippocampus, amygdala, striatum, thalamus, nucleus accumbens, lateral hooked nucleus, and dorsal raphe nucleus. In the hippocampus, GRID1 is specifically expressed in the granule cells of the dentate gyrus.

Protocols

Product Specific Protocols
WB protocol for GRID1 antibody 13040-1-APDownload protocol
IHC protocol for GRID1 antibody 13040-1-APDownload protocol
IP protocol for GRID1 antibody 13040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Anesthesiology

Single-nucleus Atlas of Sevoflurane-induced Hippocampal Cell Type- and Sex-specific Effects during Development in Mice

Authors - Shao-Yong Song
View Article