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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRIK1 Polyclonal antibody

GRIK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25779-1-AP

Synonyms

EAA3, EEA3, GLR5, GluK1, GluR 5, GLUR5, Glutamate receptor 5, GRIK1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GRIK1 Polyclonal antibody (25779-1-AP)

    WB analysis using 25779-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25779-1-AP (GRIK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using GRIK1 Polyclonal antibody (25779-1-AP)

    IHC staining of human cerebellum using 25779-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25779-1-AP (GRIK1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using GRIK1 Polyclonal antibody (25779-1-AP)

    IHC staining of human cerebellum using 25779-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25779-1-AP (GRIK1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inmouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25779-1-AP is GRIK1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRIK1 fusion protein Ag22664 相同性解析による交差性が予測される生物種
Full Name glutamate receptor, ionotropic, kainate 1
Calculated molecular weight 918 aa, 104 kDa
Observed molecular weight 95-100 kDa
GenBank accession numberBC156975
Gene symbol GRIK1
Gene ID (NCBI) 2897
RRIDAB_2880236
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GRIK1 antibody 25779-1-APDownload protocol
IHC protocol for GRIK1 antibody 25779-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Exp Ther Med

Glutamate receptor ionotropic, kainate 1 serves as a novel tumor suppressor of colorectal carcinoma and predicts clinical prognosis.

Authors - Zhong Ren
View Article
humanWB,IHC

Oncol Res

GRIK1 promotes glioblastoma malignancy and is a novel prognostic factor of poor prognosis

Authors - Guoqiang Hou
View Article