NMDAR1/GRIN1 Polyclonal antibody
NMDAR1/GRIN1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 27676-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 9 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
27676-1-AP targets NMDAR1/GRIN1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NMDAR1/GRIN1 fusion protein Ag26364 相同性解析による交差性が予測される生物種 |
Full Name | glutamate receptor, ionotropic, N-methyl D-aspartate 1 |
Calculated molecular weight | 105 kDa |
Observed molecular weight | 120 kDa |
GenBank accession number | NM_000832 |
Gene symbol | NMDAR1 |
Gene ID (NCBI) | 2902 |
RRID | AB_2880944 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for NMDAR1/GRIN1 antibody 27676-1-AP | Download protocol |
IHC protocol for NMDAR1/GRIN1 antibody 27676-1-AP | Download protocol |
IF protocol for NMDAR1/GRIN1 antibody 27676-1-AP | Download protocol |
IP protocol for NMDAR1/GRIN1 antibody 27676-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Adv RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission | ||
Aging (Albany NY) EGB761 ameliorates chronic cerebral hypoperfusion-induced cognitive dysfunction and synaptic plasticity impairment. | ||
Exp Neurol DPP-4 inhibition by linagliptin ameliorates age-related mild cognitive impairment by regulating microglia polarization in mice | ||
Heliyon Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1 | ||
Cell Discov Postsynaptic lncRNA Sera/Pkm2 pathway orchestrates the transition from social competition to rank by remodeling the neural ensemble in mPFC | ||
Life Sci Identification of a robust scoring system based on metabolic genes followed by in-depth validation of ATP1A3 in glioma |