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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRINA Polyclonal antibody (20851-1-AP)

    WB analysis using 20851-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20851-1-AP (GRINA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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GRINA Polyclonal antibody

GRINA Polyclonal Antibody for WB, ELISA
Cat No. 20851-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Glutamate [NMDA] receptor-associated protein 1, HNRGW, LFG1, Protein lifeguard 1, Putative MAPK-activating protein PM02

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20851-1-AP targets GRINA in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRINA fusion protein Ag14716 相同性解析による交差性が予測される生物種
Full Name glutamate receptor, ionotropic, N-methyl D-aspartate-associated protein 1 (glutamate binding)
Calculated molecular weight 371 aa, 41 kDa
Observed molecular weight49 kDa
GenBank accession numberBC041788
Gene Symbol GRINA
Gene ID (NCBI) 2907
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z429
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutamate Receptor Ionotropic NMDA-Associated Protein 1 (GRINA, also known as TMBIM3) is a transmembrane protein involved in calcium homeostasis and cell survival. It plays a crucial role in regulating intracellular calcium levels, which are essential for processes such as cell survival, neurotransmitter release, and apoptosis (PMID: 32124700). GRINA also involves vesicle transport and sorting, particularly in secretory cells like neurons and mammary glands (PMID: 31426446). Additionally, GRINA has been implicated in regulating aerobic glycolysis and tumor progression in gastric cancer (PMID: 30541591).

Protocols

Product Specific Protocols
WB protocol for GRINA antibody 20851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols