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  • Featured Product
  • KD/KO Validated

GRK5 Polyclonal antibody, PBS Only

GRK5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17032-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human skeletal muscle tissue using GRK5 Polyclonal antibody (17032-1-AP)

    WB analysis of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GRK5 Polyclonal antibody (17032-1-AP)

    WB analysis using 17032-1-AP (same clone as 17032-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GRK5 Polyclonal antibody (17032-1-AP)

    WB analysis using 17032-1-AP (same clone as 17032-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using GRK5 Polyclonal antibody (17032-1-AP)

    WB analysis of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using GRK5 Polyclonal antibody (17032-1-AP)

    IHC staining of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17032-1-AP (GRK5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using GRK5 Polyclonal antibody (17032-1-AP)

    IHC staining of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17032-1-AP (GRK5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using GRK5 Polyclonal antibody (17032-1-AP)

    IHC staining of mouse heart using 17032-1-AP (same clone as 17032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17032-1-AP (GRK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using GRK5 Polyclonal antibody (17032-1-AP)

    IHC staining of mouse heart using 17032-1-AP (same clone as 17032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17032-1-AP (GRK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17032-1-PBS targets GRK5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRK5 fusion protein Ag10511 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor kinase 5
Calculated molecular weight 590 aa, 68 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC064506
Gene symbol GRK5
Gene ID (NCBI) 2869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GRK5, also named as GPRK5, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. It is Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. GRK5 is crucial for proper neuronal morphogenesis and the establishment of functional neuronal circuitry.