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  • Featured Product
  • KD/KO Validated

GRK5 Polyclonal antibody, PBS Only

GRK5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17032-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GRK5 Polyclonal antibody (17032-1-AP)

    WB analysis using 17032-1-AP (same clone as 17032-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GRK5 Polyclonal antibody (17032-1-AP)

    WB analysis using 17032-1-AP (same clone as 17032-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using GRK5 Polyclonal antibody (17032-1-AP)

    WB analysis of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using GRK5 Polyclonal antibody (17032-1-AP)

    WB analysis of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using GRK5 Polyclonal antibody (17032-1-AP)

    IHC staining of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17032-1-AP (GRK5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using GRK5 Polyclonal antibody (17032-1-AP)

    IHC staining of human skeletal muscle using 17032-1-AP (same clone as 17032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17032-1-AP (GRK5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using GRK5 Polyclonal antibody (17032-1-AP)

    IHC staining of mouse heart using 17032-1-AP (same clone as 17032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17032-1-AP (GRK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using GRK5 Polyclonal antibody (17032-1-AP)

    IHC staining of mouse heart using 17032-1-AP (same clone as 17032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17032-1-AP (GRK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17032-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17032-1-PBS targets GRK5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRK5 fusion protein Ag10511 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor kinase 5
Calculated molecular weight 590 aa, 68 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC064506
Gene symbol GRK5
Gene ID (NCBI) 2869
RRIDAB_2115453
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GRK5, also named as GPRK5, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. It is Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. GRK5 is crucial for proper neuronal morphogenesis and the establishment of functional neuronal circuitry.