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- KD/KO Validated
GRK6 Polyclonal antibody
GRK6 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11439-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, human kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
11439-1-AP targets GRK6 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GRK6 fusion protein Ag1992 相同性解析による交差性が予測される生物種 |
| Full Name | G protein-coupled receptor kinase 6 |
| Calculated molecular weight | 66 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC017272 |
| Gene symbol | GRK6 |
| Gene ID (NCBI) | 2870 |
| RRID | AB_2248070 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRK6 antibody 11439-1-AP | Download protocol |
| IHC protocol for GRK6 antibody 11439-1-AP | Download protocol |
| IP protocol for GRK6 antibody 11439-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.
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