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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRP Polyclonal antibody

GRP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28482-1-AP

Synonyms

BN, gastrin releasing peptide, GRP, GRP 10, preproGRP, proGRP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GRP Polyclonal antibody (28482-1-AP)

    WB analysis using 28482-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 28482-1-AP (GRP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GRP Polyclonal antibody (28482-1-AP)

    IHC staining of human lung cancer using 28482-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28482-1-AP (GRP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GRP Polyclonal antibody (28482-1-AP)

    IHC staining of human lung cancer using 28482-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28482-1-AP (GRP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GRP Polyclonal antibody (28482-1-AP)

    IHC staining of human colon cancer using 28482-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28482-1-AP (GRP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GRP Polyclonal antibody (28482-1-AP)

    IHC staining of human colon cancer using 28482-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28482-1-AP (GRP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

28482-1-AP targets GRP in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRP fusion protein Ag29357 相同性解析による交差性が予測される生物種
Full Name gastrin-releasing peptide
Calculated molecular weight 16 kDa
Observed molecular weight16-18 kDa
GenBank accession numberBC004488
Gene symbol GRP
Gene ID (NCBI) 2922
RRIDAB_2881152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GRP antibody 28482-1-APDownload protocol
IHC protocol for GRP antibody 28482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Ann N Y Acad Sci

The von Economo neurons in the frontoinsular and anterior cingulate cortex.

Authors - John M Allman
View Article
humanIHC

Front Immunol

A novel necroptosis-related gene index for predicting prognosis and a cold tumor immune microenvironment in stomach adenocarcinoma

Authors - Muhammad Khan
View Article